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BLASE Object

BlaseData-class BlaseData
Blase Data Object
as.BlaseData()
Conversion to BlaseData
assign_pseudotime_bins()
Assign Pseudotime Bins to a source object's metadata
genes()
Get genes of a BLASE Data object.
`genes<-`()
Set genes of a BLASE Data object.
show(<BlaseData>)
Show an BlaseData object

BLASE Mapping Result Object

MappingResult()
Blase Mapping Result
`bulk_name<-`()
Set name of bulk of a BLASE Mapping Results object.
best_bin()
Get best bin of a BLASE Mapping Results object.
best_correlation()
Get best correlation of a BLASE Mapping Results object.
bootstrap_iterations()
Get the number of bootstrap iterations performed for a BLASE Mapping Results object.
bulk_name()
Get name of bulk of a BLASE Mapping Results object.
mapping_history()
Get the mapping history for a BLASE Mapping Results object.
metric()
Get the mapping history for a BLASE Mapping Results object.
strong_mapping()
Get if the result is strong for a BLASE Mapping Results object.
top_2_distance()
Get the difference in correlation between the top 2 most correlated bins for a BLASE Mapping Results object.
show(<MappingResult>)
Show an MappingResult object

Mapping Bulks

map_all_best_bins()
Map many bulk samples in the same dataframe
map_best_bin()
Map the best matching SC bin for a bulk sample

Plots

plot_bin_population()
Plot the populations of a bin
plot_mapping_result()
Plot a summary of the mapping result
plot_mapping_result_corr()
Plot a mapping result's correlation
plot_mapping_result_heatmap()
Plot a mapping result heatmap

Parameter Tuning

evaluate_parameters()
Evaluate n_bins and n_genes for bin mapping
evaluate_top_n_genes()
Evaluate Top Genes
find_best_params()
Identify the Best Parameters For Your Dataset
plot_find_best_params_results()
Plot the results of the search for good parameters

Gene Selection

calculate_gene_peakedness()
calculate_gene_peakedness
gene_peakedness_spread_selection()
Gene Peakedness Spread Selection
get_top_n_genes()
Get Top Genes From An AssociationTestResult
plot_gene_peakedness()
plot_gene_peakedness
smooth_gene()
smooth_gene

Annotation of scRNA-seq

annotate_sce()
Annotate a SCE with BLASE Mappings

Utilities

get_bins_as_bulk()
Get a pseudobulk of bins with at least 2 replicates

Data

MCA_PF_SCE
Malaria Cell Atlas Plasmodium falciparum for BLASE Vignette
painter_microarray
Painter 2018 Plasmodium falciparum 48h asexual lifecycle microarray data
tradeSeq_BLASE_example_sce
TradeSeq Example SCE for BLASE Vignette
zhang_2021_heat_shock_bulk
Zhang 2021 Plasmodium falciparum heat shock bulk data